Transcriptome study on immune response against Vibrio parahaemolyticus challenge in gill of abalone Haliotis discus hannai Ino

Abalone ( Haliotis discus hannai Ino) is one of the most vital economic shellfish cultured in China. However, bacterial and viral infection often leads to its high mortality serious losses. In this study, transcriptome sequencing was performed on gill abalone challenged by Vibrio parahaemolyticus for 0 h (control group, A0h), 8 (A8h), 48 (A48h), respectively. Each library has an average 53,361,675 clean reads. Approximately 85.71% reads were mapped reference genome Ino. comparison with A0h 7,656 2,789 genes differentially expressed A8h A48h groups, The (DEGs) mostly enriched oxidation–reduction process oxidoreductase activity from GO analysis as well immunity metabolism-related pathways Kyoto Encyclopedia Genes Genomes analysis. Cluster demonstrated that all DEGs categorized into eight subclusters adopting gene expression model. Specifically, a total 1,070 significantly downregulated two clusters related some metabolic pathways, ABC transporters, cell adhesion. Moreover, 212 upregulated subcluster several immune-related including nucleotide-binding oligomerization domain (NOD)-like receptor signaling pathway apoptosis. Some members pattern recognition receptors family, which can initiate inflammatory induce apoptosis, stimulated upregulate after challenge C1q, perlucin, low-density lipoprotein receptor–related protein. addition, caspase-dependent apoptosis inactive, whereas caspase-independent stimulating response challenge. To eliminate invading pathogen, immune effectors such interleukin-17, matrix metalloproteinase, lysozyme, superoxide, ferritin also gill. study provides more information comprehending defense mechanisms against V. abalone.